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Image Search Results
Journal: Antioxidants
Article Title: Ergothioneine-Mediated Neuroprotection of Human iPSC-Derived Dopaminergic Neurons
doi: 10.3390/antiox13060693
Figure Lengend Snippet: Derivation of day 40 dopaminergic neurons and quality control analysis. ( A ) Schematic representation of differentiation protocol to derive day-40-induced dopaminergic neurons from hiPSCs (BJ and GM23720) and hESC (H9). Scale bar= 100 µm ( B ) Confocal imaging of neuronal and dopaminergic markers. DAPI stains the nucleus. Beta-Tubulin III and NeuN are neuronal markers. Tyrosine hydroxylase (TH) is a dopaminergic marker. Scale bar = 100 µm; Scale bar for zoomed in figures = 20 µm ( C , D ) ImageJ analysis of confocal images of TH and NeuN to quantify the percentage of neurons and dopaminergic neurons present in the culture. Data are represented as mean ± SD ( n = 3). Data were analysed by unpaired Student’s t -test. *** p ≤ 0.001.
Article Snippet: Cells were permeabilised in perm buffer (Miltenyi Biotec) before blocking and primary antibody incubation of
Techniques: Control, Imaging, Marker
Journal: Antioxidants
Article Title: Ergothioneine-Mediated Neuroprotection of Human iPSC-Derived Dopaminergic Neurons
doi: 10.3390/antiox13060693
Figure Lengend Snippet: Effect of ET on cellular metabolism and tyrosine hydroxylase levels in 6-OHDA-treated SH-SY5Y cells. ( A ) ATP assay showing relative levels in intracellular ATP as compared to the control group. ( B ) Fluo-4 AM assay measures intracellular free calcium levels. Data are represented as mean ± SD ( n = 4). ( C ) Flow cytometry analysis to quantify relative amounts of tyrosine hydroxylase (TH) protein expression as compared to control. ( D ) Flow cytometry analysis to quantify relative tau neuronal protein expression among the treatment groups as compared to control. ( A – D ) (Control: no treatment; 6OHDA: 15 µM 6-hydroxydopamine; ET: 1 mM ergothioneine; VHCL: 100 µM verapamil hydrochloride). Data were analysed by one-way ANOVA with Bonferroni’s multiple comparison post hoc test. Unless stated in figures, ns: non-significant, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.
Article Snippet: Cells were permeabilised in perm buffer (Miltenyi Biotec) before blocking and primary antibody incubation of
Techniques: ATP Assay, Control, Flow Cytometry, Expressing, Comparison